DNA

Part:BBa_K3878007:Design

Designed by: Mathis LAVAUD   Group: iGEM21_UParis_BME   (2021-09-27)


Toehold_miR21_1 (to Toehold_miR21_7)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 7
    Illegal XbaI site found at 113
    Illegal SpeI site found at 149
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 149
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 7
    Illegal XbaI site found at 113
    Illegal SpeI site found at 149
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 7
    Illegal XbaI site found at 113
    Illegal SpeI site found at 149
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 274


Design Notes

This part can be used with molecular cloning into a plasmid coding for a repressor gene followed by a T7 terminator (BBa_K3878000).



Source

From IDT

References